Objective To establish a HPLC-based method for simultaneous determination of 2 classes of compounds (flavonoids and chromones) and 6 their effective components,(including prin-O-glucosylcimifugin, cimifugin, 4 -O-β-D-glucosyl-5-O-methylvisamminol, quercetin, sec-o-glucosylhamaudol and formononetin), in Yupingfeng Decoction.
目的建立以高效液相色谱法同时测定玉屏风散中2类化合物(黄酮类和色原酮类)6种有效成分升麻苷、升麻素、5-O-甲基维斯阿米醇苷、槲皮素、亥茅酚苷、芒柄花素含量的分析方法。
Results The linear range of prim-O-glucosylcimifugin,4′-O-β-D-glucosyl-5-O-methylvisamminol and cimifugin,sec-O-glucosylhamaudol were 1.
结果升麻素、升麻素苷、5-O-甲基维斯阿米醇苷、亥茅酚苷能够达到基线分离;升麻素苷和5-O-甲基维斯阿米醇苷质量浓度在1。
was determined by the chromatoptometry of molybdenum phosphotungstate-lithium sulfate:hamaudol was taken as the reference substance and molybdenum phosphotungstate-lithium as the developer.
方法采用磷钨酸钼-硫酸锂比色法测定总色原酮含量:以亥茅酚为对照品,磷钨酸钼-硫酸锂为显色剂,在pH值为10。
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