Prothrombin was activated by prothrombinase complex simulating the natural condition.
方法以牛血为原料,采用柠檬酸钡吸附、硫酸铵分级沉淀、DEAE-纤维素色谱等方法制备凝血酶原,并模拟生理条件用凝血酶原活酶复合物,包括牛凝血因子Ⅴ、Ⅹa、磷脂和Ca2+对凝血酶原进行激活。
Objective To measure various coagulation markers such as prothrombin fragment F1+2(F1+2),thrombin/antithrombin complexes(TAT) and D-dimer,and evaluate the outcome of early diagnosis and management of pre-disseminated intravascular(coa)-gulations(pre-DIC) in high-risk neonates.
目的探讨监测危重新生儿血浆凝血酶原片段F1+2、凝血酶抗凝血酶复合物(TAT)及D-二聚体等凝血及纤溶因子水平对弥散性血管内凝血前期(pre-DIC)的诊断及治疗意义。
