A molecular model for the disintegrin elegantin inferred that Thr45 amino acid residue flanking the RGD sequence in most disintegrins creates a cavity within the protein core, which maybe is important on regulating the hairpin RGD loop structure.
具体方法是:用基因点突变技术将华丽蛇毒素的K~(41)K~(42)K~(43)R~(44)T~(45)I~(46)突变成腹蛇毒素的S~(41)K~(42)A~(43)G~(44)T~(45)I~(46)和S~(41)K~(42)A~(43)G~(44)I~(46),然后分析突变蛋白的结构、性质和功能变化。
A New Snake Record in Sichuan Province——Maculophis bellus
四川蛇类一新纪录——方花丽斑蛇
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